![]() Id entification and quantification of dioscin was carried out using a Reverse Phase High Pressure Liquid Chromatography (RP-HPLC). Dioscin was extracted from 5g of freeze dried tubers for consequent identification and quantification. DNA sequences were assembled with sequencher ® and then a multiple sequence alignment through clustalW in MEGA 5 from which phylogenetic trees were drawn. DNA was extracted from lyophilized tubers and leaf sample s and Polymerase chain reaction (PCR) carried out on the DNA samples with direct sequencing of the PCR products. ![]() The study also used reverse phase High performance Liquid Chromatography (RP-HPLC) to identify and quantify dioscin content in Kenyan yams. This study used three universal molecular markers (matK, rbcL, trnL_F) to investigate the species position of cultivated Kenyan yams and identify their relatedness to major African species and to species in the gene bank. This project aimed at establishing the taxonomi c position of Kenyan yams in relation to gene bank species and West African yams using a molecular approach and the respective identification and quantification of dioscin from the tubers. ![]() The taxonomic position of cultivated yams in Kenya has been a subject of speculation over the years (Mwirigi et al, 2010). However, for dioscin quantification to be possible it was imperative to identify the taxonomic position of these yams. It is therefore essential to quantify the amounts of dioscin in Dioscorea species in Kenya. Apart from being used in steroidal hormones production, dioscin has other health promoting effects such as antioxidant effects and reduction of postmenopausal symptoms in women (Wang et al. Diosgenin is us ed in the production of steroidal hormones like progesterone (Sautour et al., 2004). On acid hydrolysis of dioscin, diosgenin is derived. Pharmacologically, Dioscorea species is known to have health promoting molecules among them the steroidal Saponin called dioscin. Dioscorea (Yams) in Kenya is a neglected crop, despite having a lot of potential as food source and as well playing a role in pharmaceutics (Mwirigi et al, 2009 ).
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